Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | FC, IH(P), IP, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2050 |
| Description | Anti-Peripheral Node Addressin (PNAd) Antibody, clone MECA-79 |
| Alternate Names |
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| Background Information | Peripheral lymph node addressin (PNAd) is a mixture of glycoproteins that is expressed on high endothelial venules (HEV) and is required for lymphocyte homing. It is shown to be a ligand for CD62L (L-selectin) that is expressed on the surface of naïve and central memory T cells. PNAd is a sulfated and fucosylated glycoprotein recognized by the prototypic monoclonal antibody, MECA-79. Naïve T cells use PNAd to traffic into lymphoid organs in non-mucosal tissue sites. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. Regulated appearance of PNAd+ HEVs is found during ontogeny of lymphoid tissues as well as in chronic inflammatory conditions. PNAd+ HEVs are reported to appear 24-48 h after birth in mouse peripheral lymph nodes and persist throughout life to support recirculation of lymphocytes. In humans PNAd+ HEVs are detected in fetal peripheral lymph nodes. PNAd+ HEVs are also shown to be present in several chronic inflammatory conditions, including thyroiditis, ulcerative colitis, psoriasis, rheumatoid synovitis, and cutaneous lymphomas. In murine models of inflammation, PNAd+ HEV-like vessels develop and these vessels are found to support lymphocyte recruitment. Studies on cancer in murine models and in humans have identified PNAd as a biomarker of improved disease prognosis and blockade of PNAd or its ligand, L-selectin, is shown to abrogate protective antitumor immunity in murine models. (Ref.: Sinha, RK et al. (2006). Vet. Immunol. Immunopathol. 110 (1-2): 97-108). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgM in PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rat monoclonal Anti-Peripheral Node Addressin (PNAd) Antibody, clone MECA-79, Cat. No. MABF2050, is tested in Flow Cytometry, Immunohistochemistry (Paraffin), , Immunoprecipitation, Inhibition, and Western Blotting for the detection of Peripheral node addressin. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Peripheral Node Addressin (PNAd) in human liver and human pancreas tissues. Western Blotting Analysis: A representative lot detected Peripheral Node Addressin (PNAd) in Western Blotting applications (Hemmerich, S., et. al. (1994). J Exp Med. 180(6):2219-26). Inhibition Analysis: A representative lot detected Peripheral Node Addressin (PNAd) in Inhibition applications (Streeter, P.R., et. al. (1988). J Cell Biol. 107(5):1853-62;Michie, S.A., et. al. (1993). Am. J. Pathol. 143(6):1688-98; Berg, E.L., et. al. (1991). J Cell Biol. 114(2):343-9). Immunoprecipitation Analysis: A representative lot detected Peripheral Node Addressin (PNAd) in Immunoprecipitation applications (Berg, E.L., et. al. (1991). J Cell Biol. 114(2):343-9). Immunohistochemistry Analysis: A representative lot detected Peripheral Node Addressin (PNAd) in Immunohistochemistry applications (Sinha, R.K., et. al. (2006). Immunology. 119(4):461-9; Streeter, P.R., et. al. (1988). J Cell Biol. 107(5):1853-62; Michie, S.A., et. al. (1993). Am J Pathol. 143(6):1688-98). Flow Cytometry Analysis: A representative lot detected Peripheral Node Addressin (PNAd) in Flow Cytometry applications (Sinha, R.K., et. al. (2006). Immunology. 119(4):461-9). |
| Biological Information | |
|---|---|
| Immunogen | Collagenase-dispersed lymph node stromal cells from BALB/c mice. |
| Clone | MECA-79 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone MECA-79 detects Peripheral Node Addressin (PNAd) in human and mouse. |
| Isotype | IgMκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Purified by ion-exchange chromatography |
| Molecular Weight | 50-250 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human tonsil tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Peripheral Node Addressin (PNAd) in human tonsil tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |