The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Pd-1 Antibody, Clone 16a2.1 Is An Antibody Against Pd-1 For Use In Western Blotting, Flow Cytometry. - Mill (Additional S&H or Hazmat Fees May Apply)
Programmed cell death protein 1 (PD-1) (UniProt Q15116), also known as CD279, hPD-1, hPD-I, hSLE1, Protein PD-1, Systemic lupus erythematosus susceptibility 2) is a type I single transmembrane protein encoded by the PDCD1 gene in human. Maintenance of peripheral tolerance by preventing the development of autoimmunity due to T lymphocytes specific for self-antigens is essential for homeostasis of the immune system. PD-1 receptor CD279 and its ligands PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC; CD273) play a vital role in the induction and maintenance of peripheral tolerance. Upregulated PD-1 expression is seen on conventional CD4+ CD25- T cells (Tconv) when exposed to mesenchymal stem cells, resulting in increased Tconv apoptosis due to PD-1 stimulation by the MSC PD-L2.
Product Information
Format
Affinity Purified
Presentation
Purified mouse monoclonal IgG2ak in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-PD-1 Antibody, clone 16A2.1 is an antibody against PD-1 for use in Western Blotting, Flow Cytometry.
Key Applications
Western Blotting
Flow Cytometry
Application Notes
Flow Cytometry Analysis: 2.0 µg of this antibody from a representative lot detected PD-1 in 1X10E6 Staph enterotoxin B treated human peripheral blood mononuclear cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the extracellular domain of human PD-1.
Epitope
Extracellular domain
Clone
16A2.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_005009
Gene Symbol
PDCD1
PD1
Purification Method
Protein G Purified
UniProt Number
Q15116
Molecular Weight
~32 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in TPA/LPS treated THP-1 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected PD-1 in 10 µg of TPA/LPS treated THP-1 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
