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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), IF, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF937 |
| Description | Anti-Notch 3 Antibody, ECD, clone 2G8 |
| Alternate Names |
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| Background Information | Neurogenic locus notch homolog protein 3 (UniProt Q9UM47; also known as Notch 3, Notch homolog 3) is encoded by the NOTCH3 (also known as CASIL, CADASIL, IMF2) gene (Gene ID 4854) in human. Notch 3 is a transmembrane receptor required for arterial differentiation and maturation of vascular smooth muscle cells in small arteries. Notch 3 is initially produced with a signal peptide (a.a. 1-39), the removal of which yields the 2282-amino acid Notch 3 (a.a. 40-2321), which undergoes a constitutive proteolytical cleavage, generating the extracellular fragment (Notch3-ECD; a.a. 40-1628) and the transmembrane/cytosolic (Notch3-TMIC; a.a. 1629-2321) fragment that remain associated at the cell surface to form a heterodimeric receptor. The ECD fragment contains all 34 EGF-like domains and the three Lin/Notch (LNR) repeats, while the extracellular portion of TMIC contains 5 ankyrin repeats. Mutations in the NOTCH3 gene have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Pathogenic mutations predominantly affect cysteine residues within EGF-like repeats of Notch3-ECD, leading to Notch3-ECD multimerization and accumulation in the tunica media of vessel walls. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Notch 3 Antibody, ECD, clone 2G8 is an antibody against Notch 3 for use in Immunohistochemistry (Paraffin), Immunofluorescence, Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 20 µg/mL of this antibody from a representative lot detected Notch 3 extracellular domain (ECD) deposits in blood vessels of CADASIL patients brain tissue sections (Courtesy of Institute for Stroke and Dementia Research, Clinical center of the Ludwig-Maximilians University Munich, Germany). Immunohistochemistry Analysis: A representative lot detected the aggregated Notch 3 extracellular domain (ECD) deposits in brain blood vessels of CADASIL patients by immunohistochemistry staining of paraformaldehyde-fixed, paraffin-embedded brain tissue sections (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96). Immunofluorescence Analysis: A representative lot detected the aggregated Notch 3 extracellular domain (ECD) deposits in brain blood vessels of CADASIL patients by fluorescent immunohistochemistry staining of paraformaldehyde-fixed frozen brain tissue sections. The Notch3-EDC immunoreactivity was seen co-localized with that of LTBP-1, but not fibrillin-1 (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96). Western Blotting Analysis: A representative lot detected an elevated accumulation of aggregated Notch 3 extracellular domain (ECD) in brain blood vessel extracts from CADASIL patients when comparing to vessel extracts from non-diseased brains (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence from the EGF-like domains 33-34 region of human Notch 3 ECD. |
| Epitope | Extracellular EGF-like domains 33-34. |
| Clone | 2G8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 2G8 reacts with unprocessed Notch 3 and the extracellular (Notch 3-ECD; a.a. 40-1628) fragment, but not the transmembrane/cytosolic fragment (Notch 3-TMIC, also called Notch 3 extracellular truncation; a.a. 1629-2321) or the Notch 3 intracellular fragment (a.a. 1662-2321). |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | 243.6 kDa (Pre-form), 239.5 kDa (mature, unprocessed), 167.13 kDa (Notch 3-ECD) calculated. ~250 kDa (Notch 3-ECD) reported (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG2aκ. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |