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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1580 |
| Description | Anti-beta-Arrestin-2 Antibody, clone 5E12.1 |
| Alternate Names |
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| Background Information | Beta-arrestin-2 (UniProt P32121; also known as Arrestin beta-2) is encoded by the ARRB2 (also known as ARB2, ARR2, BARR2) gene (Gene ID 409) in human. β-Arrestin 1 and 2 are cytosolic adapter proteins originally identified as negative regulators of G protein-coupled receptor (GPCR) signaling through receptor desensitization and internalization. It is now known that these multifunctional scaffold proteins also elicit G-protein-independent signalling pathways by linking the activated GPCRs to multiple signaling molecules, such as ERK1/2, Akt, and Src kinases. In addtion to GPCRs, β-arrestins are also involved in receptor tyrosine kinase (RTK) signalling pathways, and β-arrestin-2 is reported to link Src and Akt to insulin receptor (IR) to ensure a full Akt activation. Although β-arrestin-1 and β-arrestin-2 share a high degree of structural homology, they do display specific functions in several physiological processes. β-arrestins can adopt multiple conformations to display distinct sets of interacting domains. Recruitment of β-arrestins to a specific GPCR results in the formation of distinct β-arrestin scaffolds, allowing a high level of specificity for a given agonist. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-beta-Arrestin-2 Antibody, clone 5E12.1 is an antibody against beta-Arrestin-2 for use in Western Blotting, Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected beta-Arrestin-2 in rat testis and human cerebral cortex & liver tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence in the C-terminal region of human beta-Arrestin-2. |
| Epitope | C-terminal region. |
| Clone | 5E12.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with spliced isoforms 1, 4, and 5 reported by UniProt (P32121). Reactivity toward spliced isoforms 2 & 3 has not been determined. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~52 kDa observed. 46.11 kDa (isoform 1), 47.27 kDa (isoform 2), 45.56 kDa (isoform 3), 48.02 kDa (isoform 4), and 4439 kDa (isoform 5) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected beta-Arrestin-2 in 200 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |