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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1623 |
| Description | Anti-FGF-9 Antibody, clone 3A10.1 |
| Alternate Names |
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| Background Information | Fibroblast growth factor 9 (UniProt P31371; also known as FGF-9, GAF, Heparin-binding growth factor 9, Glia-activating factor, HBGF-9) is encoded by the FGF9 (also known as SYNS3) gene (Gene ID 2254) in human. Fibroblast growth factor 9 (FGF9) belongs to the FGF family of proteins that regulate a variety of cell functions, including proliferation, differentiation, survival, and motility, via FGF receptors (FGFRs). FGF-9 interacts with several FGFRs and binds preferentially to FGFR3. FGF-9 is highly expressed in cancer-associated fibroblasts (CAFs) in gastric cancer and may play a pivotal role in the interaction between cancer cells and their surrounding stromal cells. FGF-9 is shown to induce MMP-7 expression in AGS and MKN28 gastric cancer cells, promoting invasion and cellular anti-apoptotic activity through activation of the Erk and Akt signaling pathways. In human brain, FGF-9 is primarily expressed by neurons in the cortex, hippocampus, thalamus, cerebellum, and spinal cord, as well as by glia in the hindbrain and spinal cord. Upregulated FGF-9 expression is found in postmortem hippocampus tissues from major depressive disorder (MDD) patients, while FGF-2 is downregulated in the depressed brain. Likewise, chronic intracerebroventricular administration of FGF-9 increased both anxiety- and depression-like behaviors in rats, while FGF-9 knockdown in the dentate gyrus of the hippocampus ameliorated anxiety-like behavior. Human FGF-9 is produced with a propeptide (a.a. 1-3), which is cleaved off posttranslationally to yield the mature protein. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-FGF-9, clone 3A10.1 , Cat. No. MABN1623, is a highly specific mouse monoclonal antibody that targets FGF-9 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected FGF-9 in human cerebellum, cerebral cortex, and pancreas tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence within the C-terminal half of human FGF-9. |
| Clone | 3A10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3A10.1 targets an internal sequence conserved among bovine, human, mouse, and rat species. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Bovine, Mouse, Porcine, Rat based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~23 kDa (non-glycosylated recombinant mature hFGF-9) observed. 23.14/23.44 kDa (human mature/pro-form), 23.08/23.38 kDa (bovine mature/pro-form), 23.11/23.41 kDa (mouse & rat mature/pro-form), 23.16/23.46 kDa (porcine mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting of recombinant human FGF-9. Western Blotting Analysis: 2 µg/mL of this antibody detected 50 ng of E. coli expressed full-length recombinant human FGF-9. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |