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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, DB, ELISA, FC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1794 |
| Description | Anti-GnRH-R Antibody, clone F1G4 |
| Alternate Names |
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| Background Information | Gonadotropin-releasing hormone receptor (UniProt P30968; also known as GnRH receptor, GnRH-R, Leutinizing hormone-releasing hormone receptor, Luteinizing-releasing hormone receptor) is encoded by the GNRHR (also known as GNRHR1, GRHR, LHRHR, LRHR) gene (Gene ID 2798) in human. GnRH-R belongs to the family of seven-transmembrane G-protein coupled receptors (GPCRs). GnRH-R resides primarily in the pituitary and is responsible for eliciting the actions of GnRH (also known as Luteinizing hormone-releasing hormone or LHRH) after its release from the hypothalamus. Luteinizing hormone (LH) is then secreted from gonadotropes of the anterior pituitary into the bloodstream following pulsatile stimulation of GnRH-R by GnRH/LHRH. The canonical GnRH-R designated by UniProt (P30968-1) corresponds to a 328-amino acid sequence that includes 4 extracellular domains (a.a. 1-38, 98-115, 185-212, 233-281), 7 transmembrane segments (a.a. 39-58, 78-97, 116-137, 165-184, 213-232, 282-300, 307-326), 4 intracellular domains (a.a. 59-77, 138-164, 233-281, 327-328). Multiple GnRH-R variants and post-translationally processed forms are reported in human brain samples. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1λ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect GnRH-R using this Anti-GnRH-R Antibody, clone F1G4 validated for use in Western Blotting, Dot Blot, ELISA, Flow Cytometry, Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Dot Blot Analysis: A representative lot detected the immunogen peptide by dot blot (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56). ELISA Analysis: A representative lot detected the immobilized immunogen peptide by ELISA (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56). Flow Cytometry Analysis: A representative lot detected surface GnRH-R immunoreactivity on live human breast carcinoma T47D and ovarian carcinoma OVCAR-3 cells. Most likely due to low affinity antibody binding or unoptimized antibody concentration used, only ~50% of the T47D and ~10% of the OVCAR-3 populations were stained (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56). Immunohistochemistry Analysis: A rerepsentative lot detected GnRH-R expression on gonadotropin-producing endocrine cells (gonadotropes) in pituitary, as well as among neurons in human hippocampus and neocortex, using formalin-fixed, paraffin-embedded human tissue sections (Wilson, A.C., et al. (2006). J. Endocrinol. 191(3):651–663). Immunohistochemistry Analysis: A rerepsentative lot detected similar hippocampus GnRH-R immunoreactivity among Alzheimers diseased (AD) and age-matched control brain sections, while significanly decreased GnRH-R immunoreactivity associated with the apical dendrites of pyramidal neurons was seen in the AD brain using formalin-fixed, paraffin-embedded tissue sections (Wilson, A.C., et al. (2006). J. Endocrinol. 191(3):651–663). Immunohistochemistry Analysis: A rerepsentative lot detected GnRH-R-positive cells in the anterior pituitary, but not the posterior pituitary using frozen human brain sections. Pre-blocking with immunogen peptide abolished the antibody staining (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56). Western Blotting Analysis: A rerepsentative lot detected the expression of multiple GnRH-R variants in human cortex and pituitary tissue lysates, including the most prominent 30 kDa, 64 kDa, and 136 kDa bands. The 136 kDa band was seen significantly downregulated in the cortex, but not pituitary from Alzheimers diseased (AD) brain when compared to age-matched control (Wilson, A.C., et al. (2006). J. Endocrinol. 191(3):651–663). |
| Biological Information | |
|---|---|
| Immunogen | BSA-conjugated linear peptide corresponding to the N-terminal sequence of human GnRH-R. |
| Epitope | N-terminal extracellular domain. |
| Clone | FIG4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Specificity of clone F1G4 was demonstrated by tissue-specific staining and by immunogen peptide blocking (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56). |
| Isotype | IgG1λ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~34 kDa observed. Clone F1G4 was reported to detect multiple GnRH-R variants and posttranslationally processed forms in human brain samples (Wilson, A.C., et al. (2006). J. Endocrinol. 191(3):651–663). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human pituitary tissue lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected GnRH-R in 10 µg of human pituitary tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |