Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, B | IF, WB, IH(P), ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN2395 |
| Description | Anti-Peripherin-2 Antibody, clone 2B7 |
| Alternate Names |
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| Background Information | Peripherin-2 (UniProt: P15499; also known as Retinal degeneration slow protein) is encoded by the Prph2 (also known as Rds) gene (Gene ID: 19133) in murine species. Peripherin-2 is a multi-pass membrane glycoprotein found in the outer segment of both rod and cone photoreceptor cells. It is localized to the photoreceptor disc rim region and is required for the initial formation and subsequent maintenance of both rod and cone outer segments. It can function as an adhesion molecule for stabilization and compaction of outer segment disks and the maintenance of the curvature of the rim. Mutations Prph2 gene are reported to cause incurable retinal degeneration. Peripherin-2 can form a wide variety of covalent and non-covalent homo- and heteromeric complexes and failure to oligomerize properly can result in protein degradation and retinitis pigmentosa. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Peripherin-2, clone 2B7, Cat. No. MABN2395, is a highly specific mouse monoclonal antibody that targets Peripherin-2/RDS and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Peripherin-2 in Western Blotting applications (Stuck, M.W., et. al. (2014). Hum Mol Genet. 23(23):6260-74). Immunofluorescence Analysis: A representative lot detected Peripherin-2 in Immunofluorescence applications (Chakraborty, D., et. al. (2010). Hum Mol Genet. 19(24):4799-812). Western Blotting Analysis: A representative lot detected Peripherin-2 in Western Blotting applications (Conley, S.M., et. al. (2010). Biochemistry. 49(5):905-11). Immunocytochemistry Analysis: A representative lot detected Peripherin-2 in Immunocytochemistry applications (Conley, S.M., et. al. (2010). Biochemistry. 49(5):905-11). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of murine Peripherin-2. |
| Epitope | C-terminus |
| Clone | 2B7 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 2B7 detects peripherin-2 in murine and bovine eye. It targets an epitope within 23 amino acids from the C-terminal end. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Bovine, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 39.26 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in the outer layer of photoreceptor cells of mouse eye tissue. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Peripherin-2 in the outer layer of photoreceptor cells of mouse eye tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |