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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN394 |
| Description | Anti-PSMD9 Antibody, clone 16C5.1 |
| Alternate Names |
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| Background Information | 26S proteasome non-ATPase regulatory subunit 9 (UniProt O00233; also known as 26S proteasome regulatory subunit p27, Homolog of rat Bridge 1, Proteasome macropain non-ATPase subunit 9) is encoded by the PSMD9 gene (Gene ID 5715) in human. PSMD9 is a proteasomal assembly chaperone that forms a stable subcomplex with two AAA-ATPases, PSMC3 and PSMC6, in assisting the assembly of the 20S and 19S particles to form the holo complex. PSMD9 contains a PDZ-like domain (a.a. 108-195) that is reported to interact with a short 4- to 7-amino acid linear sequence motifs (SLIMs) at the C-terminal end of its client proteins, including the RNA-binding protein hnRNPA1, the ribosomal protein S14, the growth hormone CSH1, the transcription factor E12, and the IL6 receptor. PSMD9 and hnRNPA1 interaction is found to play a role in IκBα proteasomal degradation, where hnRNPA1 functions as shuttle receptor that recruits IκBα to the proteasome for degradation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PSMD9 Antibody, clone 16C5.1 is an antibody against PSMD9 for use in Western Blotting, Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PSMD9 in 10 µg of HepG2 cell lysate. Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected PSMD9 in normal human kidney and prostate tissues, as well as human prostate cancer tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human PSMD9 internal fragment. |
| Epitope | Internal. |
| Clone | 16C5.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with spliced isoforms p27-L and p27-S, but not spliced isoform 3. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~26 kDa observed. 24.68 kDa (isoforms p27-L) and 22.76 kDa (isoforms p27-S) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in SHSY-5Y cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected PSMD9 in 10 µg of SHSY-5Y cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |