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Millipore This Anti-Melanopsin Antibody, Clone 1f8.1 Is Validated For Use In Western Blotting, Immunohistochemistry (Paraffin) For The Detection Of Melanopsin . - Mill (Additional S&H or Hazmat Fees May Apply)
Melanopsin (UniProt Q9UHM6; also known as Opsin-4) is encoded by the OPN4 (also known as MOP) gene (Gene ID 94233) in human. Opsins belong to the superfamily of G protein-coupled receptors (GPCRs). Melanopsin is a blue light-sensitive opsin protein that is capable of rendering cells intrinsically light responsive. Melanopsin expression defines a population of retinal ganglion cells that are intrinsically photosensitive (ipRGCs). Melanopsin allows mammals to measure irradiance for centrally mediated responses such as circadian entrainment. In addition, the information generated by melanopsin is used by the visual system as a signal for light adaptation. Using melanopsin-dependent phototransduction mechanism coupled with extrinsic signals originating with rods and cones, ipRGCs can encode light levels over many decimal orders, allowing the visual system to adapt to a broad range of light intensity from starlight to broad daylight.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2aλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Melanopsin Antibody, clone 1F8.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Melanopsin .
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Melanopsin in human retina tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human Melanopsin C-terminal sequence
Epitope
Near C-terminus
Clone
1F8.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 1F8.1 is expected to react with both spliced isoforms of human melanopsin.
Isotype
IgG2aλ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_150598
Gene Symbol
OPN4
MOP
Purification Method
Protein G Purified
UniProt Number
Q9UHM6
Molecular Weight
~52 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human retinal pigment epithelia (RPE) ARPE-19 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Melanopsin in 10 µg of human retinal pigment epithelia (RPE) ARPE-19 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
