The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore This Anti-Perk Antibody, Clone 4f6.1 Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of Perk. - Mill (Additional S&H or Hazmat Fees May Apply)
Eukaryotic translation initiation factor 2-alpha kinase 3 (EC 2.7.11.1; UniProt Q9NZJ5; also known as Eukaryotic translation initiation factor 2 alpha kinase 3, HsPEK, Pancreatic EIF2-alpha kinase, PRKR-like endoplasmic reticulum kinase) is encoded by the EIF2AK3 (also known as PEK, PERK, WRS) gene (Gene ID 9451) in human. The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) induces the activation of three main unfold protein response (UPR) pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CAAT/enhancer binding protein (C/EBP) homologous protein (CHOP) upregulation.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-PERK Antibody, clone 4F6.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of PERK.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PERK in human liver tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the Internal (C-terminal half) of human PERK.
Epitope
Internal (C-terminal half)
Clone
4F6.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_004827
Gene Symbol
EIF2AK3
PEK
PERK
Purification Method
Protein G Purified
UniProt Number
Q9NZJ5
Molecular Weight
~125 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected PERK in 10 µg of HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
