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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, B | WB, IH(P), IP, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN875 |
| Description | Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 |
| Alternate Names |
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| Background Information | Gamma-aminobutyric acid (GABA) receptor subunit gamma-2 (UniProt P18507; also known as GABA(A) receptor subunit gamma-2) is encoded by the GABRG2 (also known as CAE2, ECA2, GEFSP3) gene (Gene ID 2566) in human. The GABAA receptor (GABAAR) is an ionotropic receptor and ligand-gated ion channel. Upon GABA binding and activation, the GABAA receptor selectively conducts chloride ions through its pore, resulting in hyperpolarization of the neuron. The GABAAR is a pentameric complex composed of two alpha, two beta, and one gamma subunit arranged around a central pore. Each subunit contains four transmembrane domains with both the N- and C-terminus located extracellularly. In human, there exist six types of alpha subunits (encoded by GABRA1-6), three types of beta subunits (encoded by GABRB1-3), and three types of gamma subunits (encoded by GABRG1-3). The gamma 2 subunit is initially produced with an signal peptide sequence (a.a. 1-39), which is then cleaved off to yield the mature subunit (a.a. 40 – 467). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, ELISA for the detection of GABAA. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GABAA γ2 Subunit in 10 µg of mouse brain tissue lysate. Western Blotting Analysis: 1.0 µg/mL from a representative lot detected GABAA γ2 Subunit in 10 µg of human brain tissue lysate. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GABAA γ2 Subunit in human cerebral cortex tissue. Western Blotting Analysis: A representative lot detected human GABAA gamma2 subunit cytoplasmic domain GST fusion protein, as well as endogenous GABAA gamma2 subunit in purified bovine brain GABAA receptor complex (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311). Immunoprecipitation Analysis: A representative lot immunoprecipitated GABAA receptor complex from rat cerebral cortex membrane extracts, as well as purified bovine brain GABAA receptor complex as measured by ligand-binding activity associated with the immune complex (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311). ELISA Analysis: A representative lot detected human GABAA gamma2 cytoplasmic domain GST fusion protein, but not GST (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the cytoplasmic domain of human GABAA γ2 Subunit. |
| Epitope | cytoplasmic domain |
| Clone | KC4-8A7 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with all three spliced isoforms of GABA(A) receptor gamma 2 subunit (GABRG2), but not gamma 1 (GABRG1) or gamma 3 (GABRG3) subunit. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~45 kDa observed. Molecular weight bands were observed at ~45 kDa for both rat and mouse brain tissue lysates. However, for human brain tissue lysates, we detected a molecular weight at ~58 kDa, as predicted by Uniprot. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected GABAA γ2 Subunit in 10 µg of rat brain tissue lysate. Note: For Western blotting analysis, DO NOT BOIL samples prior to electrophoresis. After dissociating receptor complex with SDS sample buffer, remove non-solublized material by centrifugation. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |