Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H, M, B, F | WB, IH(P), IP, IF | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN894 |
| Description | Anti-Synapsin-1 Antibody, clone 10.22 |
| Alternate Names |
|
| Background Information | Synapsin-1 (UniProt P17599; also known as Synapsin I) is encoded by the SYN1 gene (Gene ID 281510) in bovine species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1–2 months of life. The NH2-terminal region is divided into A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The C-terminal region contains spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn IIa and IIb, domain H in Syn IIa, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Synapsin-1 Antibody, clone 10.22 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, Immunofluorescence for the detection of Synapsin-1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synapsin-1 in human, mouse, and rat cerebral cortex tissues. Western Blotting Analysis: A representative lot detected synapsin Ia/Ib in mouse cortical neuron lysates (Medrihan, L., et al. (2013). Nat. Commun. 4:1512). Western Blotting Analysis: A representative lot detected purified bovine brain synapsin Ia/Ib (Messa, M., et al. (2010). J. Cell Sci. 123(13):2256-2265). Western Blotting Analysis: A representative lot detected synapsin-1 in Torpedo (electric ray) synaptosomal preparations and in GST-cyclophilin B pull-downs (Lane-Guermonprez, L., et al. (2005). J. Neurochem. 93(6):1401-1411). Western Blotting Analysis: A representative lot detected synapsin-1 in the same rat brain subcellular fractions as cyclophilin B (Lane-Guermonprez, L., et al. (2005). J. Neurochem. 93(6):1401-1411). Western Blotting Analysis: A representative lot detected synapsin Ia/Ib, but not IIa/IIb, in rat brain post-nuclear supernatants (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16). Immunoprecipitation Analysis: A representative lot immunoprecipitated synapsin Ia/Ib, but not IIa/IIb, from rat brain synaptosomal preparations using protein G beads with pre-bound rabbit anti-mouse IgG (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16). Immunofluorescence Analysis: Clone 10.22 ascites fluid was employed to localize synapsin-1 immunoreactivity within retinal inner plexiform layer (IPL) of floating or whole-mount rat retinas (Mandell, J.W., et al. (1992). J. Neurosci. 12(5):1736-1749). Note: Clone 10.22 does not bind significantly to protein G. For immunoprecipitation application, pre-coat protein G beads with an anti-mouse IgG antibody is recommended (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16). |
| Biological Information | |
|---|---|
| Immunogen | Purified bovine brain synapsin-1. |
| Epitope | Pro-rich domain D. |
| Clone | 10.22 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 10.22 reacts with both synapsin-1 spliced isoforms (Ia and Ib), but not synapsin-2 spliced isoform IIa or IIb (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16). |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~75 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain cytosol tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Synapsin-1 in 10 µg of rat brain cytosol tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |