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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1132 |
| Description | Anti-CDKL5 Antibody, clone 8F3.1 |
| Alternate Names |
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| Background Information | Cyclin-dependent kinase-like 5 (EC 2.7.11.22; UniProt O76039; also known as Serine/threonine-protein kinase 9) is encoded by the CDKL5 (also known as EIEE2, STK9) gene (Gene ID 6792) in human. CDKL5/STK9 is a serine/threonine protein kinase widely distributed in all tissues, with highest levels in thymus, testes, and brain, where it is found localized in the nucleus and dendrite of neurons. CDKL5/STK9 expression is found peaked in the early postnatal period and Cdkl5-knockdown in mice leads to autistic-like behavior, dendritic hypotrophy and impairment of neural circuit communication. Likewise, human CDKL5 gene mutations are linked to X-linked neurological developmental disorders involving severe mental retardation and seizures appearing in the first postnatal months. Point mutations in CDKL5 have also been reported in patients with atypical variants of Rett syndrome (RTT), infantile spasms and severe neurodevelopmental retardation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CDKL5 Antibody, clone 8F3.1 is an antibody against CDKL5 for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected CDKL5 in 10 µg of rat hippocampus tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human CDKL5 internal fragment. |
| Epitope | Internal. |
| Clone | 8F3.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with both spliced isoforms of human CDKL5 reported by UniProt (O76039). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, Rat. Predicted to react with Human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~115 kDa observed. 115.5 kDa (isoform 1) and 107.5 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse cerebellum tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected CDKL5 in 10 µg of mouse cerebellum tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |