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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1147 |
| Description | Anti-SH2D1A Antibody, clone SuGr-20 18A9 |
| Alternate Names |
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| Background Information | SH2 domain-containing protein 1A (UniProt O60880; also known as Duncan disease SH2-protein, Signaling lymphocytic activation molecule-associated protein, SLAM-associated protein, T-cell signal transduction molecule SAP) is encoded by the SH2D1A (also known as DSHP, SAP, XLP, XLP1, XLPD) gene (Gene ID 4068) in human. SAP/SH2D1A is a 628-amino acid (isoform A) adaptor protein expressed in T cells, natural killer (NK) cells, NK-T cells, and some B cells. SAP/SH2D1A is made up almost exclusively of a Src homology 2 (SH2) domain (a.a. 6-104) and plays an important role in multiple immune cell functions, including T helper 2 (TH2) cytokine production, T cell-dependent antibody production, follicular T helper (TFH) cell differentiation, NK-T cell development, CD8+ T cell- and NK cell-mediated cytotoxicity. SAP couples signaling lymphocytic activation molecule (SLAM) family receptors to tyrosine phosphorylation signals through its interaction with the SH3 domain of Src family protein tyrosine kinase Fyn. In addition, SAP can prevent SH2 domain-containing negative regulators, such as phosphatases SHIP-1 and SHP-1, from inactivating SLAM-related receptors by blocking their interaction with the receptors. Evidence also shows that SAP/SH2D1A regulates T cell-dependent antibody production and TFH cell differentiation independent of Fyn. SAP/SH2D1A gene is mutated and inactivated in X-linked lymphoproliferative disease, a rare human immunodeficiency characterized by an uncontrolled immune response to Epstein-Barr virus infection. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SH2D1A Antibody, clone SuGr-20 18A9 is an antibody against SH2D1A for use in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Recombinant full-length human SH2D1A. |
| Clone | SuGr-20 18A9 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone SuGr-20 18A9 recognizes recombinant full-length human SH2D1A spliced isoform A (UniProt O60880-1) by Western blotting. Reactivity toward spliced isoforms B to F (UniProt O60880-2 to O60880-6) has not been determined. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~14 kDa observed. 14.19 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting of recombinant human SH2D1A. Western Blotting Analysis: 0.5 µg/mL of this antibody detected 0.1 µg of recombinant human SH2D1A. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |