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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IHC, IH(P), ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1152 |
| Description | Anti-ROR2 Antibody, clone Nt 2535-2835 |
| Alternate Names |
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| Background Information | Tyrosine-protein kinase transmembrane receptor ROR2 (EC 2.7.10.1; UniProt Q9Z138; also known as mRor2, Ntrkr2, Neurotrophic tyrosine kinase receptor-related 2) is encoded by the Ror2 gene (Gene ID 26564) in murine species. Ror2 is a receptor tyrosine kinase that binds Wnt5a and negatively regulates canonical Wnt signaling. Although in C. elegans and Xenopus Ror2 is reported to modulate Wnt signaling nonautonomously by preventing Wnt from stimulating adjacent cells, studies conducted in mammalian cells suggest that Ror2 cytoplasmic domain is crucial for its function and Ror2 inhibits Wnt signaling in a cell-autonomous manner. Ror2 and the closely related Ror1 play essential roles during development. Human ROR2 gene mutations have been associated with dominant Brachydactyly B and recessive Robinow syndrome. Ror2-null mice exhibit skeletal abnormalities similar to the human syndromes. In addition, these mice display heart and lung defects and die perinatally. Ror2 is initially translated with an N-terminal signal sequence (a.a. 1-33), the removal of which yields the mature receptor with an extracellular region (a.a. 34-403), a transmembrane segment (a.a. 404-424), followed by the cytoplasmic region (a.a. 425-944) that contains the kinase domain (a.a. 473-746), a Pro-rich domain (a.a. 784-857) sandwiched between two Ser/Thr-rich domains (a.a. 753-782 and a.a. 859-882). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ROR2 Antibody, clone Nt 2535-2835 is an antibody against ROR2 for use in Western Blotting, Immunohistochemistry, Immunohistochemistry (Paraffin), ELISA. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot detected Ror2 immunoreactivity in parafffin-embedded leiomyosarcoma (LMS) tumor, lung cancer, and breast cancer tissue sections (Courtesy of Dr. Roel Nusse, Stanford University, CA). Immunohistochemistry Analysis: A representative lot detected Ror2 expression in various regions of wild-type and Ror2+/-, but not Ror2-/-, mouse E13.5 embryos by both fluorescent and non-fluorescent immunohistochemistry using paraformaldehyde-fixed, paraffin-embedded whole mount sections (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176). Immunohistochemistry Analysis: A representative lot detected Ror2 expression in the dermis of wild-type, but not Ror2-knockout mouse skin from E16.5 mouse embryos using paraformaldehyde-fixed, paraffin-embedded whole mount sections (van Amerongen, R., et al. (2012). Dev. Biol. 369(1):101-114). Western Blotting Analysis: A representative lot detected full-length murine Ror2, as well as Ror2 deletion constructs lacking the first Ser/Thr-rich domain (ST1; a.a 753-782) or the Pro-rich domain (a.a. 784-857), but not Ror2 deletion mutants lacking the ST2 (a.a. 859-882) domain (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176). Enzyme-linked Immunoabsorbent Assay (ELISA): The immunoreactivity of a representative lot toward the immunogen MBP fusion protein was confirmed by ELISA (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the second Ser/Thr-rich domain of mouse mRor2. |
| Epitope | Second Ser/Thr-rich domain. |
| Clone | Nt2535-2835 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone Nt 2535-2835 is specific to the mouse and human ROR2, but does not detect mouse ROR1. Clone Nt 2535-2835 exclusively detects ROR2 protein in paraffin-embedded tissues (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~120 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MEF-1 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected ROR2 in 10 µg of MEF-1 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |