Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, Inhibition | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1272 |
| Description | Anti-Endothelial Protein C Receptor Antibody, clone 1560 |
| Alternate Names |
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| Background Information | Endothelial protein C receptor (UniProt: Q64695; also known as EPCR, Activated protein C receptor, APC receptor, Centrocyclin, Centrosomal protein CCD41, Endothelial cell protein C receptor, CD201) is encoded by the Procr (also known as Epcr) gene (Gene ID: 19124) in murine species. EPCR is a multi-functional single-pass type I membrane protein that is expressed in endothelial cells. Its expression has also been reported in hematopoietic, neuronal, epithelial progenitor cells, and breast cancer stem cells. It binds to activated protein C and enhances protein C activation by the thrombin-thrombomodulin complex and thereby plays a role in the protein C pathway controlling blood coagulation. It is also shown to play a role in mediating activated protein C-induced cytoprotective effects, including antiapoptotic, anti-inflammatory, and barrier stabilization. EPCR exhibits sequence and 3-dimensional structural homology with the major histocompatibility class 1/CD1 family of proteins, particularly CD1d. It contains a tightly bound phospholipid in the antigen presenting groove and phosphatidylcholine is shown to be the major phospholipid bound to human EPCR. Removal of this lipid results in the loss of protein C binding. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2b in PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Endothelial Protein C Receptor, clone 1560, Cat. No. MABS1272, is a rat monoclonal antibody for detection of murine Endothelial Protein C Receptor and is tested for use in Flow Cytometry and Inhibition Assays. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected Endothelial Protein C Receptor in Flow Cytometry applications (Li, W., et. al. (2005). J Thromb Haemost. 3(7):1351-9; Balazs, A.B., et. al. (2006). Blood. 107(6):2317-21). Inhibition Analysis: A representative lot detected Endothelial Protein C Receptor in Inhibition applications (Li, W., et. al. (2005). J Thromb Haemost. 3(7):1351-9; Disse, J., et. al. (2011). J Biol Chem. 286(7):5756-67). |
| Biological Information | |
|---|---|
| Immunogen | A full length recombinant mouse endothelial protein C receptor. |
| Clone | 1560 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 1560 specifically detects endothelial protein C receptor in murine species. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 27.19 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in bEND.3 mouse endothelial cells. Flow Cytometry Analysis: 1 µg of this antibody detected Endothelial Protein C Receptor in one million bEND.3 mouse endothelial cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |