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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ICC, IHC, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1274 |
| Description | Anti-GPx4 Antibody, clone 1B4 |
| Alternate Names |
|
| Background Information | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial (EC 1.11.1.12; UniProt O70325; also known as GPx-4, GSHPx-4, mtPHGPx, PHGPx, Phospholipid hydroperoxide glutathione peroxidase mitochondrial, snGPx, Sperm nuclei glutathione peroxidase) is encoded by the Gpx4 gene (Gene ID 625249) in murine species. Gpx4 is a member of the glutathione peroxidase (GPx) family of peroxidases that protect cells against oxidative damage by reducing lipid hydroperoxides to their corresponding alcohols and by reducing free hydrogen peroxide to water. Eight glutathione peroxidase members (GPx1-8) have been identified in human. Inducible Gpx4-knockout in transgenic mice reveals the essential role of Gpx4 in maintaining proper kidney function, and Gpx4 depletion results in massive cell death of renal tubular epithelia in vivo. While the most abundant mammalian GPx, GPx1, that forms tetramers, GPx4 exists in the monomeric form. Three alternatively spliced GPx4 isoforms originate from the same Gpx4 gene, a cytosolic (also called the short form), a mitochondrial (also called the long form), and a nuclear form. The short form is expressed ubiquitously in embryonic and adult tissues in varying degrees, while the expression of the other two forms is restricted to testis where they exert significant functions during male gametogenesis. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GPx4 Antibody, clone 1B4 is an antibody against GPx4 for use in Immunocytochemistry, Immunohistochemistry, Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected downregulated GPx-4 expression upon hydroxytamoxifen/Tam treatment of MERCreMER-expressing mouse embryonic fibroblasts (MEFs) PFa1(flox/flox)-MerCreMer whose last three Gpx4 exons were flanked by loxP sites (Seiler, A., et al. (2008). Cell Metab.;8(3):237-248). Western Blotting Analysis: A representative lot detected downregulated GPx-4 expression in kidney tissue extracts from tamoxifen/Tam-fed transgenic mice (CreERT2;Gpx4fl/fl) that express Cre-ERT2 and have both Gpx4 alleles flanked by loxP sites (Friedmann Angeli, J.P., et al. (2014). Nat. Cell Biol.16(12):1180-1191). Western Blotting Analysis: A representative lot detected downregulated GPx-4 expression upon hydroxytamoxifen/Tam treatment of lung fibroblasts derived from transgenic mice (CreERT2;Gpx4fl/fl) that express Cre-ERT2 and have both Gpx4 alleles flanked by loxP sites (Friedmann Angeli, J.P., et al. (2014). Nat. Cell Biol.16(12):1180-1191). Western Blotting Analysis: A representative lot detected high GPx-4 expression in both primary (HRPEitC) and transformed (HK-2) human tubular epithelial kidney cells (Friedmann Angeli, J.P., et al. (2014). Nat. Cell Biol.16(12):1180-1191). Immunohistochemistry Analysis: A representative lot detected GPx-4 expression in paraformaldehyde-fixed, paraffin-embedded mouse testis sections (Courtesy of Dr. Marcus Conrad, Helmholtz Zentrum München, Germany). Immunohistochemistry Analysis: A representative lot detected downregulated GPx-4 expression in paraformaldehyde-fixed, paraffin-embedded testis sections from GPx-4-knockout mice when compared to wild-type controls (Schneider, M., et al. (2009). FASEB J. 23(9):3233-3242). Immunocytochemistry Analysis: A representative lot detected downregulated GPx-4 expression in paraformaldehyde-fixed, Triton X-100-permeablized epididymal spermatozoa from Gpx4-knockout mice when compared to wild-type controls by fluorescent immunocytochemistry (Schneider, M., et al. (2009). FASEB J. 23(9):3233-3242). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminal sequence of mouse GPx-4. |
| Epitope | C-terminus |
| Clone | 1B4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Reacts with the Mitochondrial (UniProt O70325-1), Cytoplasmic (UniProt O70325-2), and Nuclear (Q91XR9-1) isoforms. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~19 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in MEF1 cells. Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected GPx-4 in MEF1 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |