Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IHC, EM | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1283 |
| Description | Anti-MLN64/STARD3 Antibody, clone 2BE-2F4 |
| Alternate Names |
|
| Background Information | MLN64/StARD3 (also known as es64, StAR-related lipid transfer protein 3, Metastatic lymph node gene 64 protein, Metastatic lymph node protein 64, StARD3, START domain-containing protein 3, Steroidogenic acute regulatory protein related; UniProt Q14849) is encoded by the MLN64 (or CAB1 & STARD3) gene in human (NCBI Gene ID 10948). MLN64/StARD3 is a late endosome (LE) multitransmembrane protein that plays an important role in intracellular cholesterol transport and steroidogenesis. In addition to binding cholesterol via its C-terminal STAR domain, MLN64/StARD3 is reported to interact with ER-anchored VAP proteins via a conserved motif called "two phenylalanines in an acidic tract" or FFAT, thereby forming an LE-ER tethering complex at the inter-organelle membrane contacts sites (MCSs), allowing heterologous membrane apposition and the exchange of metabolites (PMID 23709693 and 24105263). Clone 2BE-2F4 is also known as 2BE2F4 and mAbMLN64-Ct, it detects an epitope within the c-terminal region encompassing the last 20% of MLN64 sequence. |
| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Mouse monoclonal IgG1κ ascites with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MLN64/STARD3 Antibody, clone 2BE-2F4 is an antibody against MLN64/STARD3 for use in Western Blotting and Immunocytochemistry, Immunohistochemistry, Electron Microscopy. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected MLN64/STARD3 in MCF7/MLN64 cells (Alpy, F., et al. (2001). J. Biol. Chem. 276:4261-4269). Immunocytochemistry Analysis: A representative lot detected MLN64/STARD3 in BT-474, MCF7, and transfected COS-1 cells (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191). Immunohistochemistry Analysis: A representative lot detected MLN64/STARD3 in human breast tissue (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191). Electron Microscopy Analysis: A representative lot detected MLN64/STARD3 in HeLa cells (Holtta-Vuori, M., et al. (2005). Molecular Biology of the Cell. 16:3873-3886). Western Blotting Analysis: A representative lot detected MLN64/STARD3 in COS-1 cell lysate (Watari, H., et al. (1997). Proc. Natl. Acad. Sci. USA. 94:8462-8467). Western Blotting Analysis: A representative lot detected MLN64/STARD3 in various human cell lines (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191). |
| Biological Information | |
|---|---|
| Immunogen | Ovalbumin-conjugated linear peptide corresponding to human MLN64/STARD3 near the C-terminus. |
| Epitope | Near C-terminus |
| Clone | 2BE-2F4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| Molecular Weight | ~50 kDa observed. This protein has isoforms at ~48 and ~49 kDa, which are covered by this antigen. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in SK-OV-3 cell lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected MLN64/STARD3 in 10 µg of SK-OV-3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |