The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Detect Mgat2 Using This Mouse Monoclonal Anti-Mgat2 Antibody, Clone 4a5.2, Cat. No. Mabs1703, Validated For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
Background Information
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC 2.4.1.143; UniProt Q10469; also known as Beta-1,2-N-acetylglucosaminyltransferase II, GlcNAc-T II, GNT-II, Mannoside acetylglucosaminyltransferase 2, MGAT2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyl-transferase II) is encoded by the MGAT2 (also known as CDG2A) gene (Gene ID 4247) in human. MGAT2 is a Golgi enzyme that catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. It possesses the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. It is highly expressed in small intestine in mice and humans. Mutations in MGTA2 gene are known to cause congenital disorders of glycosylation 2A, which is a multi-system disorder caused by defective glycoprotein biosynthesis. It leads to under-glycosylated levels of serum glycoproteins that result in defects in neuronal development, coagulation disorders, hypotonia, and immunodeficiency.
Ref.: Tan, J., et al. (1995). Eur. J. Biochem. 231, 317-328. Tan J., et al. (1996), Am. J. Hum. Genet. 159, 810-817.
Product Information
Format
Purified
Presentation
Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect MGAT2 using this mouse monoclonal Anti-MGAT2 Antibody, clone 4A5.2, Cat. No. MABS1703, validated for use in Western Blotting.
Key Applications
Western Blotting
Biological Information
Immunogen
GST-tagged internal fragment from the C-terminal half of human MGAT2.
Clone
4A5.2
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 4A5.2 targets an internal epitope within the C-terminal half of human MGAT2.
Isotype
IgG2aκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002399
Gene Symbol
MGAT2
CDG2A
Purification Method
Protein G purified.
UniProt Number
Q10469
Molecular Weight
~52 kDa observed. 51.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in T47D cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected MGAT2 in 10 µg of T47D cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
