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Millipore This Anti-Modified Citrulline Antibody Is Validated For Use In Western Blotting And Elisa For The Detection Of Modified Citrulline. - Mill (Additional S&H or Hazmat Fees May Apply)
Citrulline is thought to be an unincorporated amino acid that plays a part in intermediary metabolism. It is produced in the small intestine by enterocytes. Citrulline concentration in plasma and serum is thought to be a reliable and noninvasive biomarker of small intestine function and mass during disease. Citrulline has also been shown to play an important role in protein homeostasis. Modified citrulline is a chemically modified form of citrulline that is more immunogenic.
Product Information
Format
Purified
Presentation
Human recombinant monoclonal IgG1λ in buffer containing PBS with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Modified Citrulline Antibody is validated for use in Western blotting and ELISA for the detection of Modified Citrulline.
Key Applications
Western Blotting
ELISA
Application Notes
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Modified Citrulline in 10 µg of treated HL-60 cell lysate, using Anti-Human secondary antibody. ELISA Analysis: A representative lot detected Modified Citrulline in ELISA.
Biological Information
Immunogen
Citrulline-containing peptide modified with 2,3-butanedione monoxime and antipyrine.
Clone
C4
Concentration
Please refer to lot specific datasheet.
Host
Human Recombinant
Isotype
IgG1λ
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Purification Method
Protein A Purfied
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in treated cornified human cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Modified Citrulline in 10 µg of treated cornified human cell lysate, using Anti-Human secondary antibody.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.