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Millipore This Anti-Hgprt, Clone 13h11.1 Is Validated For Use In Western Blotting For The Detection Of Hgprt. - Mill (Additional S&H or Hazmat Fees May Apply)
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8; UniProt P00492; also known as HGPRT, HGPRTase) is encoded by the HPRT1 (also known as HGPRT, HPRT) gene (Gene ID 3251) in human. HPRT is a purine salvage enzyme that catalyzes the condensation of 5-phosphoribosyl 1-pyrophosphate (PRPP) and hypoxanthine or guanine to form inosine 5-monophosphate (IMP) and guanosine 5-monophosphate (GMP), respectively. PRPP is an essential compound for the de novo pathway of purine synthesis as well as the synthesis of pyrimidine compounds. PRPP concentrations in erythrocyte are markedly increased in individuals with HPRT deficiency. Complete HPRT deficiency leads to Lesch–Nyhan disease (LND) characterized by uric acid overproduction, motor dysfunction, cognitive disability, and self-injurious behavior. Partial deficiency of HPRT activity causes Lesch–Nyhan variant (LNV) characterized by hyperuricemia and variable degree of neurological manifestations, depending on the residual enzyme activity.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-HGPRT, clone 13H11.1 is validated for use in Western Blotting for the detection of HGPRT.
Key Applications
Western Blotting
Biological Information
Immunogen
GST-tagged human HGPRT recombinant fragment lacking N- and C-terminal regions.
Epitope
Internal
Clone
13H11.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000185
Gene Symbol
HPRT1
HPRT
Purification Method
Protein g Purified
UniProt Number
P00492
Molecular Weight
~25 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HGPRT in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
