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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT165 |
| Description | Anti-p130cas/BCAR1 Antibody, clone 2C1.1 |
| Alternate Names |
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| Background Information | Breast cancer anti-estrogen resistance protein 1 (UniProt P56945; also known as Cas scaffolding protein family member 1, Crk-associated substrate, p130Cas) is encoded by the BCAR1 (also known as CAS, CAS1, CASS1, CRKAS) gene (Gene ID 9564) in human. p130Cas/BCAR1 is an adaptor protein that belongs to the CAS family of scaffold proteins, members of which also include Nedd9, HEF-1 (CAs-L), EFS, and CASS4. Structurally, p130Cas is composed of a Src-homology 3 (SH3) domain, an adjacent large substrate-binding domain containing 15 repetitions of the YxxP motif, a main site of tyrosine phosphorylation on the p130Cas/BCAR1 molecule that generates SH2-binding sites, a proline and serine rich region, and a highly conserved four-helix bundle (focal adhesion targeting [FAT] domain). p130Cas/BCAR1 plays an important role in transducing signaling originated from mechanical stimuli, extracellular matrix (ECM) adhesion, as well as growth factor stimulation cascades during normal development and diseases. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human p130cas/BCAR1 C-terminal fragment. |
| Epitope | C-terminal region. |
| Clone | 2C1.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with all eight spliced isoforms of human p130Cas reported by UniProt (P56945-1 through P56945-8). |
| Isotype | IgMκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Purified by Ion-Exchange Chromatography |
| UniProt Number |
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| Molecular Weight | ~93 kDa observed. 93.14-97.88 kDa (isoforms 1-3 and isoforms 5-8) and 77.73 kDa (isoform 4) calculated. Fully phosphorylated target band migrates with an apparent molecular size of ~130 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected p130cas/BCAR1 in 10 µg of A431 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |