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Millipore This Anti-Alpha-Tubulin Antibody, Clone Rm113 Is Validated For Use In Western Blotting, Immunocytochemistry For The Detection Of Alpha-Tubulin. - Mill (Additional S&H or Hazmat Fees May Apply)
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Product Information
Format
Purified
Presentation
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected alpha-Tubulin in A431 cells.
Biological Information
Immunogen
A peptide corresponding to the C-terminus of α-Tubulin.
Epitope
C-terminus
Clone
RM113
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
TUBA1B
Purification Method
Protein A Purfied
UniProt Number
P68363
Molecular Weight
~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected alpha-Tubulin in A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
