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Millipore This Anti-Beta-Actin Antibody, Clone Rm112 Is Validated For Use In Western Blotting, Immunocytochemistry For The Detection Of Beta-Actin. - Mill (Additional S&H or Hazmat Fees May Apply)
Actin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show > 90% amino-acid (aa) homology between isotypes and > 98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.
Product Information
Format
Purified
Presentation
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.
Biological Information
Immunogen
A peptide corresponding to the N-terminus of beta-Actin.
Epitope
N-terminus
Clone
RM112
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
ACTB
Purification Method
Protein A Purfied
UniProt Number
P60709
Molecular Weight
~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
