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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT529 |
| Replaces | 04-1109 |
| Description | Anti-Integrin beta-1 (CD29) Antibody, clone 13C4.1 |
| Alternate Names |
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| Background Information | Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Integrin beta-1 (CD29), clone 13C4.1, Cat. No. MABT529, is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of Integrin beta-1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Integrin beta-1 in human breast cancer, cerebral cortex, and lung tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human Integrin beta-1 fragment corresponding to the membrane-proximal extracellular region. |
| Clone | 13C4.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 13C4.1 targets an epitope within the membrane-proximal extracellular region present in all five spliced isoforms of human Integrin beta-1 reported by UniPort (P05556). |
| Isotype | IgG1λ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~150 kDa observed. Target band size appears larger than the calculated molecular weights of 88.42/87.45/91.62/91.03/88.88 kDa (isoform Beta-1A/Beta-1B/Beta-1C/Beta-1C-2/Beta-1D pro-form) and 86.19/85.22/89.40/88.81/86.66 kDa (mature isoform Beta-1A/Beta-1B/Beta-1C/Beta-1C-2/Beta-1D) due to glycosylation. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in U937 cell lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Integrin beta-1 in 10 µg of U937 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |