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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT533 |
| Replaces | 04-1074 |
| Description | Anti-PECAM-1 Antibody, clone 4A8.1 |
| Alternate Names |
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| Background Information | Platelet endothelial cell adhesion molecule (UniProt P16284; also known as CD31, EndoCAM, GPIIA, PECA1, PECAM-1) is encoded by the PECAM1 gene (Gene ID 5175) in human. PECAM-1 (CD31) is a 130 kDa cell adhesion molecule that is expressed at the junctions of all continuous endothelia, as well as most bone marrow-derived hematopoietic cells, including platelets, monocytes, granulocytes and some lymphocytes. The human PECAM1 and murine Pecam1 genes both produce multiple spliced isoforms with varying lengths in cytoplasmic domain, with the isoform Long being the major form expressed in human tissues and endothelial cells (ECs), and isoform delta 14-15 being the predominantly expressed murine form. PECAM-1 plays an import role in maintaining vascular integrity and function by mediating ECs interactions with pericytes and smooth muscle cells (SMCs). PECAM-1 is initially produced with a signal peptide (human a.a. 1-27, murine a.a. 1-17) sequence, the removal of which yields the mature protein with a large extracellular region (human a.a. 28-601, mouse a.a. 18-590) composed of six C2-type Ig-like domains, followed by a transmembrane seqment (human a.a. 602-620, mouse a.a. 591-609) and a cytoplasmic tail (human a.a. 621-738, mouse a.a. 610-727) that plays an essential role in PECAM-1-mediated cellular signaling. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-PECAM-1 Antibody, clone 4A8.1, Cat. No. MABT533, is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of PECAM-1 (CD31). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PECAM-1 in human cerebellum, lung, and pancreas tissue sections. Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PECAM-1 in 10 µg of Jurkat cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human PECAM-1 cytoplasmic domain. |
| Epitope | cytoplasmic domain |
| Clone | 4A8.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4A8.1 was raised against the cytoplasmic domain of PECAM-1 (CD31) isoform Long. Reactivity toward spliced isoforms Delta12, Delta13, Delta14, Delta14-15, and Delta15 with various cytomplasmic domain deletions is possible, but has not not been confirmed. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~130 kDa observed. Target band size appears larger than the calculated molecular weights of 79.58/77.52/77.25/77.46/76.30/78.42 kDa (mature isoform Long/Delta12/Delta13/Delta14/Delta14-15/Delta15) and 82.54/80.48/80.21/80.42/79.26/81.38 kDa (isoform Long/Delta12/Delta13/Delta14/Delta14-15/Delta15 pro-form) due to glycosylation. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in THP-1 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected PECAM-1 in 10 µg of THP-1 human acute monocytic leukemia cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |