Sigma-Aldrich Bacillus Cereus Selective

Quality Level

200

Composition

Polymyxin B sulfate, 50000 (IU)

Packaging

pkg of 10 vials

Technique(S)

microbiological culture: suitable

Application(S)

food and beverages
microbiology

Compatibility

GranuCult^®Mannitol egg yolk polymyxin agar (base) (1.05267)

Storage Temp.

2-8°C

Suitability

selective by defined nutrients forBacillus cereus

Inchi

1S/C48H82N16O13.H2O4S/c1-27(2)24-37-47(76)59-32(11-19-52)41(70)56-31(10-18-51)43(72)61-35(14-22-65)39(68)54-21-13-34(45(74)57-33(12-20-53)44(73)64-38(48(77)63-37)25-28-6-4-3-5-7-28)60-42(71)30(9-17-50)58-46(75)36(15-23-66)62-40(69)29(8-16-49)55-26-67;1-5(2,3)4/h3-7,26-27,29-38,65-66H,8-25,49-53H2,1-2H3,(H,54,68)(H,55,67)(H,56,70)(H,57,74)(H,58,75)(H,59,76)(H,60,71)(H,61,72)(H,62,69)(H,63,77)(H,64,73);(H2,1,2,3,4)

Inchi Key

HNDFYNOVSOOGDU-UHFFFAOYSA-N

Related Categories

Microbial Culture Media

General Description

Bacillus cereusSelective Supplement contains Polymyxin B sulfate in lyophilized form. It suppresses the growth of accompanying bacterial flora during culturingBacillus cereus.

Application

Bacillus cereusSelective Supplement is an additive for the preparation of MYP Agar Base for the enumeration ofBacillus cereusfrom food and animal feed.

Linkage

Replaces: 1.09875

Analysis Note

Growth promotion test in accordance to ISO 11133.
Inoculum on reference medium
- Bacillus cereus ATCC 11778 (WDCM 00001):
10 - 100
- Bacillus subtilis ATCC 6633 (WDCM 00003): 10 - 100
Recovery on test medium
- Bacillus cereus ATCC 11778 (WDCM 00001):
≥ 70 %
- Bacillus subtilis ATCC 6633 (WDCM 00003): no limit
Colony colour
- Bacillus cereus ATCC 11778 (WDCM 00001):
pink
- Bacillus subtilis ATCC 6633 (WDCM 00003): yellow
Precipitate
- Bacillus cereus ATCC 11778 (WDCM 00001):
+
- Bacillus subtilis ATCC 6633 (WDCM 00003): -
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
Incubation: Bacillus 24-48 hrs. 30°c aerobic; other strains 48 hrs. 30°CGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, GermanyGHS07H302P264 - P270 - P301 + P312 - P501