Empirical, a Fortis LS Co. Fastaq Hotstart Dna Polymerase (1000 Units)

Description

Performance

Empirical’s FASTaq HotStart DNA Polymerase is a chemically modified hot-start Taq polymerase designed to reduce non-specific DNA amplification during reaction set up.

FASTaq HotStart DNA Polymerase and Buffer System has been developed for Automated Dispensing, High-Throughput and Microarray applications.

FASTaq HotStart DNA Polymerase has activation times of two to 10 minutes at 95°C, resulting in faster cycling and fewer program changes.

Chemical modification keeps FASTaq HotStart DNA Polymerase free of any contaminating DNA from modifying agents (such as antibodies). FASTaq DNA Polymerase, IVD Grade, is manufactured under ISO13485 Quality System and is suitable for use as a raw material in IVD applications.

Applications:

• HotStart PCR and RT-PCR
• Labeling of DNA with modified nucleotides
• Manufacture of application mixes requiring stringent controls and validation

Features:

• High Processivity
• Purity: >98% Pure by SDS-Page
• Concentration: >5U/ul
• Free from Bacterial DNA: 60 qPCR Cycles Minimum
• Free from Human Genomic Contamination: 60 qPCR Cycles Min.
• Unspecific endonucleases (?DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
• Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
• Animal-derived additives: None
• Activation Time: 2 – 10 Minutes (Assay Dependent)

Shipped in:

Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20, 0.2% (v/v); glycerol, 50% (v/v); pH 9.0 at +25°C

Shipped With:

10 FASTaq Reaction Buffer