Revvity Insulin (Human) Lance Ultra Tr-Fret Detection K

Revvity The Lance® Ultra Human Insulin Detection Kit Is Designed For Detection And Quantitation Of Human Insulin In Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. Insulin Is Synthesized As A Proinsulin Hormone Of 110 Aa By Beta-Cells Of The Islets Of Langherans In The Pancreas. After Removal Of The Precursor Signal Peptide, Proinsulin Is Post-Translationally Cleaved Into Two Chains (Peptide A Of 21 Aa And Peptide B Of 30 Aa) That Are Covalently Linked Via Two Disulfide Bonds And Secreted Upon Increased Glucose Concentration In Blood. Blood Concentration Increases From Around 50 Pmol/L To 300-400 Pmol/L 30 Min After Glucose Uptake. Insulin Is A Key Player In The Control Of Both Carbohydrate And Lipid Metabolism And Has Been Implicated In Various Diseases Including Diabetes, Heart Disease And Obesity.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)