Revvity Tnf (H) Lance Ultra Tr-F

Revvity The Lance®Ultra Human Tnf Detection Kit Is Designed For Detection And Quantitation Of Human Tnf In Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. Tumor Necrosis Factor Alpha (Tnf) Is A Multifunctional Proinflammatory Cytokine Synthesized Mainly By Nucleated Blood Cells As A 233 Aa Type Ii Transmembrane Protein Which Is Cleaved By Adam17 Between Aa 76-77 To Form A Soluble Homotrimeric Complex. Tnf Plays A Role In Lipid Metabolism, Coagulation, And Endothelial Function And Has Been Associated With Cancer, Infection And Inflammation (Including Inflammatory Bowel Disease), Ischemia/Reperfusion Injury And Heart Failure, And Insulin Resistance - PEGSK (Additional S&H Or Hazmat Fees May Apply)