Revvity Il-6 (Human) Lance Ultra Tr-Fret Detection Kit

Revvity The Lance® Ultra Human Il-6 Detection Kit Is Designed For Detection And Quantitation Of Human Interleukin-6 In Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. The 500 Point Kit Contains Enough Reagents To Run 500 Wells In 384-Well Format, Using A 20 Μl Reaction Volume (15 Μl Of Sample). The 5,000 Point Kit Contains Enough Reagents To Run 5,000 Wells In 384-Well Format, Using A 20 Μl Reaction Volume (15 Μl Of Sample). No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. Interleukin 6 (Il-6) Is A ~22 Kda Pleiotropic Cytokine That Acts Not Only On The Immune System, But Also Affects Many Physiological Events In Various Organs. Il6 Exerts Pro- Or Anti-Inflammatory Effects, Depending On The Target Cell Analyzed And The In Vivo Environmental Circumstances. Il-6 Is A Differentiation And Proliferation Factor For B And T Cells, And Acts As A Migration Factor On Monocytic Cells. It Is The Major Activator Of Acute-Phase Protein Expression In The Liver, A Hematopoietic Factor, And Acts As A Survival Factor On Neuronal Cells. Il-6 Signals Through Binding To The Gp130/ Il-6r Receptor Complex, Leading To The Activation Of Jak/Stat, Mapk And Pi3k Cascades.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)