Revvity Il-8 (Human) Lance Ultra Tr-Fret Detection Kit

Revvity The Lance® Ultra Human Il-8 Detection Kit Is Designed For Detection And Quantitation Of Human Interleukin 8 In Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. The 500 Point Kit Contains Enough Reagents To Run 500 Wells In 384-Well Format, Using A 20 Μl Reaction Volume (15 Μl Of Sample). The 5,000 Point Kit Contains Enough Reagents To Run 5,000 Wells In 384-Well Format, Using A 20 Μl Reaction Volume (15 Μl Of Sample). No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. Interleukin 8 (Il8 Or Cxcl8), A Member Of The Elr+ Cxc Chemokine Family, Is A 8.4 Kda Polypeptide That Forms Homodimers In Vivo. Il8 Is Secreted By Several Types Of Cells: Fibroblasts, Monocytes, Macrophages And Endothelial Cells, Among Many Others, In Response To Inflammatory Stimuli. It Is A Chemoattractant And Activator For Neutrophils, Directing Them From Periferal Blood To The Site Of Inflammation. It Is Also A Potent Angiogenic Factor Promoting Endothelial And Epithelial Migration And Proliferation In Several Cancers, And Is Associated With Metastasis. It Signals Through Two Specific G Protein-Coupled Receptors, Cxcr1 And Cxcr2, Sharing ~77% Identity.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)