Revvity CCL4 (human) LANCE Ultra TR-FRET

Revvity The Lance®Ultra Human Ccl4/Mip-1 Detection Kit Is Designed For Detection And Quantitation Of Human Ccl4/Mip-1 In Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. C-C Motif Chemokine 4 (Ccl4), Also Known As Macrophage Inflammatory Protein-1beta (Mip-1), Is A 69 Amino Acid Chemokine Signaling Through The Ccr5 Receptor. It Is A Chemoattractant For Natural Killer Cells, Monocytes, And A Variety Of Other Immune Cells. Ccl4 Is Also An Important Regulator Of Macrophage Migration. Ccl4 Is Rapidly Synthesized By Activated T Cells, B Cells, And Monocytes. It Is Also Produced By Neutrophils After Adhesion To The Laminin Contained In The Basement Membrane Of Blood Vessels. It Is One Of The Major Hiv-Suppressive Factors Produced By Cd8+ T-Cells And Has Been Reported To Show A Direct Antiviral Activity Against Hsv-1. Recombinant Ccl4 Also Induces A Dose-Dependent Inhibition Of Different Strains Of Hiv-1, Hiv-2, And Simian Immunodeficiency Virus - PEGSK (Additional S&H Or Hazmat Fees May Apply)