Revvity Pcsk9 (Human) Lance Ultra Tr-Fret Detection Kit

Revvity The Lance Ultra Human Pcsk9 Detection Kit Is Designed For Detection And Quantitation Of Human Pcsk9 In Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance And Lance (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. Human Proprotein Convertase Subtilisin / Kexin Type 9 (Pcsk9) Belongs To The Proteinase K Subfamily Of The Secretory Subtilase Family. This Glycoprotein Contains 692 Amino Acids, Including A Signal Peptide, A Prodomain, And A Catalytic Domain. Initially Synthesized As A Soluble 74 Kda Precursor Protein, It Is Cleaved Into 14 Kda And 60 Kda Domains, Which Remain Associated. This Protein Plays A Major Regulatory Role In Cholesterol Homeostasis And Is Highly Expressed In The Kidney, Liver, And Intestine. Pcsk9 Binds To The Low-Density Lipoprotein Receptor (Ldlr), Inducing Ldlr Degradation. Reduced Ldlr Levels Result In Decreased Metabolism Of Low-Density Lipoprotein, Which Could Lead To Hypercholesterolemia. Inhibition Of Pcsk9 Function Is Currently Being Explored As A Means Of Lowering Cholesterol Levels. Mutations In This Gene Have Been Associated With A Rare Form Of Autosomal Dominant Familial Hypercholesterolemia.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)