Revvity Il-11 (Human) Lanceultratr-Fret Detection Kit

Revvity The Lance® Ultra Human Il-11 Detection Kit Is Designed For Detection And Quantitation Of Human Interleukin-11 In Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. Human Interleukin 11 (Il11) Is A Non-Glycosylated Protein Of 23 Kda (179 Amino Acids). It Is Produced By Bone Marrow Stromal Cells (Fibroblasts) And Also By A Variety Of Mesenchymal Cells. Il11 Stimulates Megakaryocytopoiesis, Activates Osteoclasts, Inhibits Epithelial Cell Proliferation And Apoptosis, And Inhibits Macrophage Mediator Production. Il-11 Also Possesses Anti-Inflammatory Activity, And Has Been Proposed As A Therapeutic Agent In The Treatment Of Chronic Inflammatory Diseases, Such As Crohns Disease And Rheumatoid Arthritis. Il11 Activity Is Mediated By The Il-11 Receptor (Il-11r) That Has Been Reported In A Wide Variety Of Cells And Tissues. Il11 Binds To The Il-11r Protein Alone With Low Affinity; The Affinity Increases When Il-11r Is Associated With Is Signal Transducer: Gp130.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)