Revvity Host Cell Residual Dna Contamination Lance Ultr

Revvity The Lance® Ultra Residual Dna Contamination Detection Kit Is Designed For Detection And Quantitation Of Contaminating Dna In Buffered Solution And Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. Biologics Can Be Impacted Negatively By Contamination With Dna Introduced During Fermentation And Purification Processes. While The Use Of Serum-Free Media In The Manufacturing Process Significantly Improved The Success Rate On Preventing Dna Impurities, Other Routes Of Contamination, Such As Microbial Contamination, Still Remain A Concern. It Is Thus Critical To Remove And Monitor Dna Impurities At Each Step In The Purification Process. This Kit Is Designed To Quantify The Levels Of Dna (From Different Hosts, Either Double- Or Single-Stranded And Of Varying Fragment Sizes) In Cell Culture Supernatants.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)