Revvity Pd-L1 (Human) Lance Ultra Tr-Fret Detection Kit

Revvity The Lance® Ultra Human Pd-L1 Detection Kit Is Designed For Detection And Quantitation Of Human Pd-L1 In Buffered Solution And Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. Programmed Death Ligand 1 (Pdl-1), Also Known As Cluster Of Differentiation 274 (Cd274) Or B7 Homolog1 (B7-H1) Belongs To The Growing B7 Family Of Immune Proteins And Has Been Demonstrated To Play A Role In The Regulation Of Immune Responses And Peripheral Tolerance. Human Pdl-1 Is Constitutively Expressed In Several Organs Such As Heart, Skeletal Muscle, Placenta And Lung, And In Lower Amounts In Thymus, Spleen, Kidney And Liver. Pdl-1, Together With Pdl-2, Are Two Ligands For Pd-1 (Programmed Death 1), A Member Of The Cd28 Family Of Immunoreceptors. By Binding To Pd-1 On Activated T-Cells And B-Cells, Pdl-1 May Inhibit Ongoing T-Cell Responses By Inducing Apoptosis And Arresting Cellcycle Progression. Accordingly, It Leads To Growth Of Immunogenic Tumor Growth By Increasing Apoptosis Of Antigen Specific T Cells And May Contribute To Immune Evasion By Cancers. Pdl-1 Thus Is Regarded As Promising Therapeutic Target For Human Autoimmune Disease And Malignant Cancers.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)