Revvity Bcl-2 (Human) Lance Ultra Tr-Fret Detection Kit

Revvity The Lance® Ultra Human Bcl-2 Detection Kit Is Designed For Detection And Quantitation Of Human Bcl-2 In Buffered Solution And Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. Bcl-2 (B-Cell Lymphoma 2) Is An Oncogenic Protein That Inhibits Apoptosis. It Is A Protein With A Mw Of 24-26 Kda, Located Within The Mitochondrial Membrane, Endoplasmic Reticulum And Nuclear Envelope. Bcl-2 Is Considered An Important Antiapoptotic Protein And Is Thus Classified As A Proto-Oncogene. Bcl-2 Overexpression Has Been Implicated In A Wide Variety Of Malignancies Including (But Not Limited To) Breast, Prostate, Skin, Colon And Pancreatic Cancers As Well As Neurodegenerative And Autoimmune Diseases. Related Anti-Apoptotic Proteins Include Bcl-Xl, Mcl-1 And Bcl-W. Proapoptotic Proteins In The Bcl-2 Family Include Bax, Bad, Bak, Blk And Bid.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)