Revvity IFN- (mouse) LANCE Ultra TR-FRET

Revvity The Lance®Ultra Mouse Ifn- Detection Kit Is Designed For Detection And Quantitation Of Mouse Interferon In Cell Culture Media Using A Homogeneous Tr-Fret (No-Wash Steps, No Separation Steps) Assay. The 500 Point Kit Contains Enough Reagents To Run 500 Wells In 384-Well Format, Using A 20 Μl Reaction Volume (15 Μl Of Sample). The 5,000 Point Kit Contains Enough Reagents To Run 5,000 Wells In 384-Well Format, Using A 20 Μl Reaction Volume (15 Μl Of Sample). No-Wash Steps, No Separation Steps Tr-Fret Technology Sensitive Detection High Reproducibility Faster Time-To-Results Easy Automation 96-Well, 384-Well, And 1536-Well Formats Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore (Ulight™ Dye). Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. In The Mouse, Interferon-Gamma (Ifn-) Is A Homodimeric Glycoprotein Of 133 Amino Acids. Interferons (Ifns) Were Discovered Due To Their Antiviral Effects. In Humans, There Are Three Families Of Ifns: Ifn Type I (Ifn-, , , , And ), Ifn Type Ii (One Single Representative, Ifn-), And Ifn Type Iii (Ifn-1-3). Antigens And Mitogens Stimulate The Production Of Ifn- In Natural Killer And Activated Helper T Lymphocytes. Ifn- Shows Multiple Effects. It Induces The Production Of Cytokines, Upregulates The Expression Of Class I And Ii Mhc Antigens And Leukocyte Adhesion Molecules. It Also Activates Macrophages And Enhances The Secretion Of Immunoglobulins By B Cells. Response To Ifn- Is Mediated By The Heterodimeric Ifn- Receptor. Importantly, Ifns Have Proven To Be Effective In The Treatment Of Several Viral Infections And Cancers - PEGSK (Additional S&H Or Hazmat Fees May Apply)