Revvity Phospho-Tbk1 (Ser172) Lance Ultra Tr-Fret Detec

Revvity Please Note Control Lysates Are Sold Separately, Catalog Number Trf4031s. Formats: Our 500 Assay Point Kit Allows You To Run 500 Wells In 384-Well Format, Using A 20 Μl Reaction Volume. Our 10,000 Assay Point Kit Allows You To Run 10,000 Wells In 384-Well Format, Using A 20 Μl Reaction Volume. Lance® And Lance® (Lanthanide Chelate Excite) Ultra Are Homogeneous (No Wash) Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer) Technologies. One Antibody Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Antibody Is Labeled With An Acceptor Fluorophore [Ulight™ Dye]. Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm. Data Are Represented As Ratiometric (665/615 Nm X 10,000). Tank-Binding Kinase 1 (Tbk1) Is Auto-Phosphorylated At Ser172. It Contributes To Multiple Pathways. Activation Of Tbk1 Leads To The Activation Of The Tlr3/4 And Cgas-Stng Signaling Pathways. Ultimately Activation Of Tbk1 Leads To Irf3 Activation. Phosphorylation Of Optineurin And P62 By Tbk1 Marks Proteins To Be Directed To The Autophagosome.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)