Revvity LSDX LANCE Eu-W1024-ITC Chelate and Eu Standard; 2 mg

Revvity LSDX LANCE Eu-W1024-ITC Chelate is optimized for the labeling of proteins and peptides at least one primary aliphatic amino group. The labeled compound can be used in homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assays (LANCE assays). Isothiocyanate (ITC) activated chelates react primarily with free amine groups such as ones a N-terminus of protein or lysine residues.  LANCE W1024 Series chelates are preferred in most HTS applications due to their lower fluorescent background in TR-FRET assays.  For most LANCE applications the W1024 series of Eu chelates with active group can be used. Usable pH range 5-11. Under neutral conditions W1024 remains stable in EDTA concentrations up to 20 mmol/L. Allophycocyanin (APC)- or ULight-labeled compounds can be used as acceptors for LANCE TR-FRET assays. LANCE (Lanthanide chelate excite) and LANCE Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight dye, allophycocyanin (APC)]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (10 nm). This results in the emission of light at 665 nm (for ULight dye and APC). - PEMSD