Revvity IL-1 (human) LANCE Ultra TR-FRET

Revvity The LANCEUltra Human IL-1 Detection Kit is designed for detection and quantitation of human interleuukin-1 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay. The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 uL reaction volume (15 uL of sample). The 5,000 point kit contains enough reagents to run 5,000 wells in 384-well format, using a 20 uL reaction volume (15 uL of sample). No-wash steps, no separation steps TR-FRET technology Sensitive detection High reproducibility Faster time-to-results Easy automation 96-well, 384-well, and 1536-well formats LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (10 nm). This results in the emission of light at 665 nm. IL1 and IL1 are central players of the immune response, displaying roles in inflammation both at local and systemic levels. Despite they seem to display very similar functions, these proteins are encoded by two independent genes sharing only 30% identity. IL1 is synthesized as a 31 kDa precursor that is cleaved by Caspase-1 (ICE) into the active 17 kDa form, and eventually released into the extracellular space. Its production has been reported in many cell types including brain and, importantly, monocytic and periferal blood mononuclear cells. After binding to its receptor, IL-1RI, IL1 triggers a cascade of kinase signaling pathways that lead to the activation of transcription factors like NFB and AP-1, eventually activating the expression of genes such as MIP-2 and C-reactive protein. - PEMSD (Additional S&H or Hazmat Fees May Apply)