Revvity IFN- (mouse) LANCE Ultra TR-FRET

Revvity The LANCEUltra Mouse IFN- Detection Kit is designed for detection and quantitation of mouse interferon in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay. The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 uL reaction volume (15 uL of sample). The 5,000 point kit contains enough reagents to run 5,000 wells in 384-well format, using a 20 uL reaction volume (15 uL of sample). No-wash steps, no separation steps TR-FRET technology Sensitive detection High reproducibility Faster time-to-results Easy automation 96-well, 384-well, and 1536-well formats LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (10 nm). This results in the emission of light at 665 nm. In the mouse, Interferon-gamma (IFN-) is a homodimeric glycoprotein of 133 amino acids. Interferons (IFNs) were discovered due to their antiviral effects. In humans, there are three families of IFNs: IFN type I (IFN-, , , , and ), IFN type II (one single representative, IFN-), and IFN type III (IFN-1-3). Antigens and mitogens stimulate the production of IFN- in Natural Killer and activated helper T lymphocytes. IFN- shows multiple effects. It induces the production of cytokines, upregulates the expression of class I and II MHC antigens and leukocyte adhesion molecules. It also activates macrophages and enhances the secretion of immunoglobulins by B cells. Response to IFN- is mediated by the heterodimeric IFN- receptor. Importantly, IFNs have proven to be effective in the treatment of several viral infections and cancers. - PEMSD (Additional S&H or Hazmat Fees May Apply)