Revvity IL-6 (mouse) LANCE Ultra TR-FRET Detection Kit; 500 Assay Pts

Revvity The LANCE Ultra Mouse IL-6 Detection Kit is designed for detection and quantitation of murine IL-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay. The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 uL reaction volume (15 uL of sample). The 5,000 point kit contains enough reagents to run 5,000 wells in 384-well format, using a 20 uL reaction volume (15 uL of sample). No-wash steps, no separation steps TR-FRET technology Sensitive detection High reproducibility Faster time-to-results Easy automation 96-well, 384-well, and 1536-well formats LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (10 nm). This results in the emission of light at 665 nm. In the mouse, Interleukin 6 (IL6) is a 187 amino acid cytokine. In humans, IL6 is produced by a variety of cell types, including T-cells, mast cells, and monocytes. IL6 signals through binding to the gp130/ IL6R receptor complex. IL6 acts not only on the immune system, but also affects many physiological events in various organs. IL6 exerts pro- or antiinflammatory responses, depending on the target cell analyzed and the in vivo environmental circumstances. IL6 is a differentiation and proliferation factor for B and T cells, and acts as a migration factor on monocytic cells. It is the major activator of acute-phase protein expression in the liver and acts as a survival factor on neuronal cells. Elevated serum IL6 levels have been observed during bacterial and viral infection, trauma, autoimmune disease, inflammation, and cancer. - PEMSD