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GenScript Cho-K1/Mrgprx2 Stable Cell Line; 2vials
List Price $8,925.00 Your Price $8,925.00
GenScript Cho-K1/Mrgprx2 Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-M00425
MFG.PART: M00425
UNSPSC: 41105322
Manufacturer: GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cells stablyoverexpress mas-related G-protein coupled receptor member X2 (MRGPRX2). MRGPRX2couples to both Gi and Gq alpha subunits in mast cells naturally, so that thePLC signaling can be triggered within the cells by agonist binding. This cellline is recommended for agonist screening and functional validations withcalcium flux assay. |
| Applications | Agonist screening and functional validations of MRGPRX2. |
Product Introduction
| Expressed Gene | Codon Optimized from NM_054030; no expressed tags |
| Target Protein | NP_473371 |
| Host Cell | CHO-K1 |
| Quantity | 2 vials of frozen cells (>1X106 pervial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately uponreceipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative.The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit(Cat. No. LT07-318, Lonza). |
| Stability | Stable through more than 15 passages with nosignificant changes in assay performance or expression profile. |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 45% culture medium, 45% FBS (Cat. No. 10099-141,Gibco), 10% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco),10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco),10% FBS (Cat. No. 10099-141, Gibco), 200 μg/ml Zeocin (Cat. No. R250-01, LifeTechnologies) |
Background
| Gene Synonyms | MRGPRX2; MRGX2 |
| Background | MRGPRX2 (also known as MRGX2) is a Gq and Gi-coupled GPCR that binds to several peptides,including the neuropeptide cortistatin-14 and the proadrenomedullin N-terminalpeptide (PAMP). It is probably involved in the function of nociceptive neuronsand regulation of nociceptor function and/or development, including thesensation or modulation of pain. Dorsal root ganglia and adrenal chromaffincells express MRGPRX2,and binding of these ligands to MRGPRX2 is thought to play a role in nociception and catecholamine release fromthe adrenal glands. |
| SKU | GSCRPT-M00425 |
|---|---|
| Supplier Part Number | M00425 |
| UM | EA |
| UNSPSC | 41105322 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |