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GenScript Cho-K1/Glp1r/Gα15 Stable Cell Line; 2vials
List Price
$8,925.00
Your Price
$8,925.00
GenScript Cho-K1/Glp1r/Gα15 Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00451
MFG.PART:
M00451
UNSPSC:
41105322
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cells stably overexpress human glucagon-like peptide 1 receptor (GLP1R) and Gα15 subunit. Activated GLP1R stimulates the adenylyl cyclase pathway naturally. While the co-expression of GLP1R and Gα15 subunit makes activated GLP1R also triggers PLC signaling and then stimulates intracellular calcium mobilization. This cell line is recommended for screening and functional validating agonists of GLP1R. |
| Applications | Screening and functional validation of agonists of GLP1R |
Product Introduction
| Expressed Gene | Codon optimized from NM_002062; no expressed tags |
| Target Protein | NP_002053 |
| Host Cell | CHO-K1/Gα15 |
| Quantity | 2 vials of frozen cells (> 1×106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability | Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 45% Culture medium, 45% FBS (Cat. No. 10099-141, Gibco), 10% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Hams F-12K (Kaighns) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | Hams F-12K (Kaighns) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen) |
Background
| Gene Synonyms | GLP-1R |
| Background | GLP1R binds specifically the glucagon-like peptide-1 (GLP-1) and has much a lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes. GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which overexpress GLP1R display improved memory and learning. |
| SKU | GSCRPT-M00451 |
|---|---|
| Featured | No |
| Supplier Part Number | M00451 |
| UM | EA |
| UNSPSC | 41105322 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |