Takara Mate & Plate Library - Universal Arabidopsis (Normalized) \5 x 1 mL

This yeast two-hybrid library was constructed from mRNA isolated from 11Arabidopsistissues, mixed in equal quantities and transformed into yeast strain Y187. The cDNA was normalized prior to library construction to reduce the copy number of abundant cDNAs derived from highly represented mRNAs, thereby increasing the representation of low copy number transcripts. The normalization process combines a Duplex-Specific Nuclease (DSN) treatment and SMARTtechnology, reduces the number of clones that must be screened in your yeast two-hybrid assay, and facilitates the identification and characterization of novel protein-protein interactions. The library was transformed into yeast strain Y187 and can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold, for screening.

Required Products

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.