Takara Lenti-X Concentrator \100 mL

The Lenti-X Concentrator is a complete reagent for the concentration of infectious lentiviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes used.

Concentrate lentivirus from any volume or from any lentiviral titer.Lentiviral supernatant was diluted into 250 mL and then concentrated down to 2.5 mL using Lenti-X Concentrator (Panel A). 10-fold serial dilutions of a high-titer lentiviral supernatant (high, medium, and low) were concentrated from a volume of 10 mL down to 100 µl using the Lenti-X Concentrator Reagent (Panel B). Titrations were performed using HT1080 cells and flow cytometry 48 hr post-transduction.