Takara Lenti-X Accelerator \400 uL

Lenti-X Accelerator is a magnetic bead-based technology designed to accelerate lentiviral transduction experiments. Traditional lentiviral transductions require overnight incubation of your cells and virus with Polybrene, but Lenti-X Accelerator allows you to complete this process in only 30 minutes and avoid using Polybrene altogether. Charged magnetic beads bind to virus particles from your lentiviral packaging supernatant and are pulled into contact with your cells in the presence of a magnetic field. A Magnetic Separator for Cell Culture (Cat. No. 631255) is available separately, or supplied together with the Lenti-X Accelerator reagent in the Lenti-X Accelerator Starter Kit (Cat. No. 631254). Lenti-X Accelerator is excellent for transducing lentivirus, MMLV retrovirus, and MSCV retrovirus preparations.

Transduction with Lenti-X Accelerator versus Polybrene.Placing virus-bound magnetic beads in a magnetic field greatly increases the localized virus concentration at the surface of the cell monolayer. This reduces the transduction time to just 5 min after a 20 min preincubation to bind the beads to the virus—compared to transduction overnight with Polybrene. Accelerated transduction also limits exposure of your sensitive target cells to viral supernatant.

Lenti-X Accelerator (25 min) is faster than Polybrene (16 H) for lentiviral transduction.A ZsGreen1-expressing Lenti-X vector was used to transduce HT1080 cells. Lenti-X Accelerator beads (8 µl) were preincubated for 20 min at room temperature with 200 µl (approx. 1x106total IFU) of viral supernatant and applied to HT1080 cells on a Magnetic Separator for 5 min. HT1080 cells were also transduced with the same amount of virus in the presence of 6 µg/mL Polybrene, for 5 min or overnight. After the cultures were grown for an additional 72 hr at 37°C, the number of transduced cells was determined by flow cytometry