Takara AAVpro Purification Kit Midi (All Serotypes) \4 Preps

The AAVpro Purification Kit Midi (All Serotypes) is a four-prep kit that can be used for purifying AAV particles from cell lysates without the need for ultracentrifugation. The purification yields highly pure AAV particles that can be used directly for in vivo transduction or transduction of cultured cells. Cryonase Cold-Active Nuclease is included. This product allows for purification of four virus preps, each from a culture size of 1 x T225 flask or 4 x 10 cm dishes.

Gene transduction of iPSC-derived cardiomyocytes using AAV1, 2, 5, and 6.AAV1, 2, 5, and 6 vectors carrying theZsGreen1gene were purified with the AAVpro Purification Kit Midi (All Serotypes) kit. Each purified vector was used for viral transduction of iPSC-derived cardiomyocytes at 500,000 vector genomes per cell. After 72 hr of infection, ZsGreen1 fluorescence was analyzed by microscopy, with an exposure time of 0.2 sec. AAV5 was additionally exposed at 0.5 sec. NC = negative control.